Specialized Tools for Electrophysiology and Cell Biology Research

(RC-25, RC-25F) Open Diamond Bath Imaging Chambers for Round Coverslips

Open diamond bath recording chambers designed for round coverslips.

  • Designed for physiological measurements of cell cultured coverslips
  • Applications include patch clamp, intracellular/extracellular recordings, and imaging
  • Uses 12 and 15 mm diameter round coverslips as the chamber floor
  • Diamond shaped bath provides laminar solution flow
  • Small bath volume facilitates fast solution exchange

The design of the RC-25 and RC-25F chambers incorporates a diamond-shaped bath which has been shown to produce laminar flow. Since the bath volumes of these chambers is small (90 and 133 ul/mm for the RC-25 and RC-25F, respectively), fluid exchange times are measured in seconds even when flow rates are less than 1 ml/min.

RC-25 Chamber

The RC-25 works equally well with either 12 or 13 mm diameter coverslips while the RC-25F uses 15 mm coverslips. In general, these coverslips are used to form the bottom of the chamber bath. NOTE: These chambers are not designed for insertion of coverslips from the top.

The RC-25 requires a platform (PM-3PH-3 or P-3) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.

 

The RC-25 is supplied with a package of #1.5 thickness glass coverslips (CS-12R, 12 mm diameter, 100/pkg) and 10 ft of PE-160 tubing.

RC-25F Chamber

The RC-25F is similar in design to the RC-25 but uses 15 mm coverslips. In general, these coverslips are used to form the bottom of the chamber bath. NOTE: These chambers are not designed for insertion of coverslips from the top.

The RC-25F requires a platform (PM-4, PH-4 or P-4) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.

 

The RC-25F is supplied with a package of #1.5 thickness glass coverslips (CS-15R, 15 mm diameter, 100/pkg) and 10 ft of PE-160 tubing.

  RC-25 RC-25F
Material polycarbonate polycarbonate
Bath dimensions
(L x W x H)
25 x 12 x 4.1 mm 24 x 13 x 4.1 mm
Volume, by depth 90 μl/mm 133 μl/mm
Bottom coverglass 12 mm round 15 mm round
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160) 1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD) 1.67 mm (OD)

 

 

Order No. Model No. Product Description USA Price Order
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0712 CS-12R15 Round cover glass, #1.5 thickness, 12 mm, 100 pack Login Login
64-0713 CS-15R15 Round cover glass, #1.5 thickness, 15 mm, 100 pack Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. Zonisamide block of cloned human T-type voltage-gated calcium channels
    Matar N, Jin W, Wrubel H, Hescheler J, Schneider T, Weiergräber M
    Epilepsy Res. 2009 Feb;83(2-3):224-34. Epub 2009 Jan 4
  2. Structural and Functional Analysis of the HCV p7 Protein
    Saint N, Montserret R, Chipot C, Penin F
    Methods Mol Biol. 2009;510:125-43
  3. Extracellular nucleotides stimulate Cl– currents in biliary epithelia through receptor-mediated IP3 and Ca2+ release
    Dutta AK, Woo K, Doctor RB, Fitz JG, Feranchak AP
    Am J Physiol Gastrointest Liver Physiol. 2008 Nov;295(5):G1004-15. Epub 2008 Sep 11
  4. Fluid flow induces mechanosensitive ATP release, calcium signalling and Cl? transport in biliary epithelial cells through a PKC?-dependent pathway
    Woo K, Dutta AK, Patel V, Kresge C, Feranchak AP
    J Physiol. 2008 Jun 1;586(Pt 11):2779-98. Epub 2008 Apr 3
  5. Activation of Cl– Channels by Human Chorionic Gonadotropin in Luteinized Granulosa Cells of the Human Ovary Modulates Progesterone Biosynthesis
    Olivero P, Leiva-Salcedo E, Devoto L, Stutzin A
    Endocrinology. 2008 Sep;149(9):4680-7. Epub 2008 May 22
  6. State-dependent calcium mobilization by urotensin-II in cultured human endothelial cells
    Brailoiu E, Jiang X, Brailoiu GC, Yang J, Chang JK, Wang H, Dun NJ
    Peptides. 2008 May;29(5):721-6. Epub 2008 Jan 31
  7. Imaging pHluorin-Based Probes at Hippocampal Synapses
    Royle SJ, Granseth B, Odermatt B, Derevier A, Lagnado L
    Methods Mol Biol. 2008;457:293-303
  8. Role of Angiotensin II Type 1A Receptor Phosphorylation, Phospholipase D, and Extracellular Calcium in Isoform-specific Protein Kinase C Membrane Translocation Responses
    Aleksandra Policha, Noriko Daneshtalab, Lina Chen, Lianne B. Dale, Christophe Altier, Houman Khosravani, Walter G. Thomas, Gerald W. Zamponi, and Stephen S. G. Ferguson
    J. Biol. Chem., Vol. 281, Issue 36, 26340-26349, September 8, 2006
  9. A novel role for protein phosphatase 2A in receptor-mediated regulation of the cardiac sarcolemmal Na+/H+ exchanger NHE1
    Andrew K. Snabaitis, Richard D’Mello, Semjidmaa Dashnyam, and Metin Avkiran1
    THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 281, NO. 29, pp. 20252–20262, July 21, 2006
  10. Translocation of Synaptically Connected Interneurons across the Dentate Gyrus of the Early Postnatal Rat Hippocampus
    Yury M. Morozov, Albert E. Ayoub, and Pasko Rakic
    The Journal of Neuroscience, May 10, 2006 • 26(19):5017–5027
  11. Molecular and Morphological Heterogeneity of Neural Precursors in the Mouse Neocortical Proliferative Zones
    Jonathan S. Gal, Yury M. Morozov, Albert E. Ayoub, Mitali Chatterjee,1 Pasko Rakic, and Tarik F. Haydar
    The Journal of Neuroscience, January 18, 2006 • 26(3):1045–1056
  12. Cyclic nucleotide regulation of store-operated Ca2 influx in airway smooth muscle
    Binnaz Ay, Adeyemi Iyanoye, Gary C. Sieck, Y. S. Prakash and Christina M. Pabelick
    Am J Physiol Lung Cell Mol Physiol 290:278-283, 2006
  13. Store-operated Ca2 Influx in Airway Smooth Muscle Interactions between Volatile Anesthetic and Cyclic Nucleotide Effects
    Y. S. Prakash, M.D., Ph.D., Adeyemi Iyanoye, M.D., Binnaz Ay, M.D., Gary C. Sieck, Ph.D., Christina M. Pabelick, M.D.
    Anesthesiology 2006; 105:976–83
  14. Integration of K+ and Cl currents regulate steady-state and dynamic membrane potentials in cultured rat microglia
    Evan W. Newell, Lyanne C. Schlichter
    The Journal of Physiology Volume 567 Issue 3 Page 869-890, September 2005
  15. Four-Dimensional Migratory Coordinates of GABAergic Interneurons in the Developing Mouse Cortex
    Ang ES Jr, Haydar TF, Gluncic V, Rakic P
    J Neurosci. 2003 Jul 2;23(13):5805-15

Order No. Model No. Product Description USA Price Order
64-0107 TA-29 Cable with bead thermistor Login Login
64-1563 PM-4 PM-4 Platform for Series 20 chambers, magnetic clamps, heated Login Login
64-1562 PM-3 PM-3 Platform for Series 20 chambers, magnetic clamps, heated Login Login
Heater Controllers
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
Accessories and Replacement Parts
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-3018 PM-plate Pressure plates for PM Series platforms, 2 pack Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
Chambers
64-0233 RC-25F Round coverslip recording chamber for 15 mm round coverslips Login Login
64-0232 RC-25 Round coverslip recording chamber for 12 or 13 mm round coverslips Login Login
Standard Dimension Coverslips: #1.5 Thickness (0.17 ± 0.02 mm)
64-0712 CS-12R15 Round cover glass, #1.5 thickness, 12 mm, 100 pack Login Login
64-0713 CS-15R15 Round cover glass, #1.5 thickness, 15 mm, 100 pack Login Login
Platforms Without Heating Elements
64-0280 P-4 P-4 Platform for Series 20 chambers, non-heater Login Login
64-0279 P-3 P-3 Platform for Series 20 chambers, non-heater Login Login
Platforms With Heating Elements
64-0287 PH-4 PH-4 Platform for Series 20 chambers, heater Login Login
64-0286 PH-3 PH-3 Platform for Series 20 chambers, heater Login Login
Valve Control Systems
64-0135 VC-66CS Perfusion valve control system, complete, with pinch valves, 6 channel Login Login
64-0185 VC-8P Perfusion valve control system, complete, with pinch valves, 8 channel Login Login
64-1940 DWV Dedicated Workstation Vacuum Login Login

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