Specialized Tools for Electrophysiology and Cell Biology Research

(RC-46SLP, RC-46SNLP, RC-47FSLP) Slotted Bath Chambers

Designed for multiple successive experiments requiring the fast exchange of #1 or #1.5 round coverslips.

  • Dual O-ring seal system enables quick coverslip exchange that's ideal for rapid screening assays
  • Slotted bath for applications requiring rapid perfusion
  • Low profile design permits low entry angle for electrodes
  • Platinum electrodes available for applications requiring electric field stimulation
  • Anodized aluminum base insures good heat transfer
  • When used with QE-1 Quick Exchange Platform, both temperature control and perfusion are possible

The RC-40 Series chambers are designed to meet the need for rapid screening of cells grown on coverslips. Their innovative design uses O-rings to seal the coverslip to the polycarbonate chamber. Therefore little or no silicone grease is needed and mechanical fasteners are not required for coverslip mounting. The chamber design consists of two parts; a polycarbonate chamber and a 40 mm diameter aluminum base. The height for these low profile chambers is 4.9 mm at their edges and tapered from its sides. O-rings are used to seal the coverslip to the polycarbonate chamber and to hold the chamber securely in the aluminum base.

Slotted Bath Chambers (RC-46SLP, RC-46SNLP, RC-47FSLP)

 

The RC-46SLPRC-46SNLP, and RC-47FSLP chambers all use a 25 mm round coverslip in a slotted bath design to provide rapid solution exchange. The RC-47FSLP chamber also has two 11.5 mm height sections for the leads from its Pt wire electrodes and its perfusion and aspiration ports.

The low volume of these slotted bath chambers and the natural laminar flow allows rapid exchange of solution, facilitating drug application studies while minimizing disturbance of attached cells. These chambers are supplied with one box of #1.5 thickness 25 mm coverslips and spare O-rings.

The RC-47FSLP chamber includes a pair of platinum wires epoxied onto the parallel sides of the oval shaped bath. The connecting wires are terminated in 1 mm pins. This model includes a perfusion inlet port and a 16 gauge adjustable suction tube. An agar bridge well has been added to accommodate a bath ground electrode. Additionally the RC-47FSLP is also supplied with 10 feet of PE-160polyethylene tubing. Electric field stimulation is achieved by use of the optional STG or SIU-102 Stimulus Isolation Unit.

Series 40 chambers are designed to be compatible with Warner's QE-1 Quick Exchange Platform and DH-35iL and DH-40iL culture dish incubators which are compatible with Warner Series 20 microscope stage adapters.

A temperature controller TC-344C or TC-324C that connects to the heated platform QE-1 or the microincubators DH-35iL and DH-40iLcan be added to set and maintain a certain temperature.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

Chambers are supplied with one box of #1.5 thickness, 25 mm coverslips and spare O-rings. Additionally, the RC-47FSLP is supplied with 10 feet of PE-160 polyethylene tubing.

 

Series 40 Chamber Slot Width Slot Length Coverslip Working Volume OD
RC-46SLP 6.5 mm 18.9 mm 25 mm round 118 µl/mm 40 mm
RC-46SNLP 4.0 mm 18.9 mm 25 mm round 75 µl/mm 40 mm
RC-47FSLP 6.5 mm 18.5 mm 25 mm round 118 µl/mm 40 mm
Order No. Model No. Product Description USA Price Order
64-0705 CS-25R Round cover glass, #1 thickness, 25 mm, 100 pack Login Login
64-0715 CS-25R15 Round cover glass, #1.5 thickness, 25 mm, 100 pack Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-1497 OR-7 Replacement o-ring for QR/RC-47FSLP, 5 pack, red Login Login
64-0351 AR-6 Adapter ring kit for DH-35i or QE-1, fits Willco, Corning, and Falcon dishes, set of 6 Login Login
89-0706 STG4002-1.6mA Stimulus generator, 2-channel, 4000 Series, ±1.6 mA Login Login
89-0707 STG4002-160µA Stimulus generator, 2-channel, 4000 Series, ±160 µA Login Login
89-0708 STG4002-16mA Stimulus generator, 2-channel, 4000 Series, ±16 mA Login Login
64-1424 SIU-102 Bipolar stimulator, high current, non-isolated Login Login
64-1425 CC-102 Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) Login Login
64-1492 OR-2 Replacement Retaining Ring o-ring for all RC-40 Series, 5 pack, black Login Login
64-1496 OR-6 Replacement o-ring for QR/RC-46SNLP, 5 pack, red Login Login
Order No. Model No. Product Description USA Price Order
64-0372 RC-46SLP Quick change chamber for 25 mm coverslips, slotted bath Login Login
64-0373 RC-46SNLP Quick change chamber for 25 mm coverslips, narrow slotted bath Login Login
64-0388 DH-40iL Culture dish incubation system Login Login
64-0349 DH-35iL Culture dish incubation system Login Login
64-0375 QE-1 Quick change platform, heated base, for RC-40 and QR-40 series chambers Login Login
64-0110 DH-35 35 mm culture dish heater Login Login
64-1921 SA-40PS1 Adapter Ring for Pecon Heating Insert P Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
89-0706 STG4002-1.6mA Stimulus generator, 2-channel, 4000 Series, ±1.6 mA Login Login
89-0707 STG4002-160µA Stimulus generator, 2-channel, 4000 Series, ±160 µA Login Login
89-0708 STG4002-16mA Stimulus generator, 2-channel, 4000 Series, ±16 mA Login Login
64-1424 SIU-102 Bipolar stimulator, high current, non-isolated Login Login
64-0705 CS-25R Round cover glass, #1 thickness, 25 mm, 100 pack Login Login
Accessories and Replacement Parts
64-0715 CS-25R15 Round cover glass, #1.5 thickness, 25 mm, 100 pack Login Login
64-1496 OR-6 Replacement o-ring for QR/RC-46SNLP, 5 pack, red Login Login
64-1497 OR-7 Replacement o-ring for QR/RC-47FSLP, 5 pack, red Login Login
64-1492 OR-2 Replacement Retaining Ring o-ring for all RC-40 Series, 5 pack, black Login Login
64-0351 AR-6 Adapter ring kit for DH-35i or QE-1, fits Willco, Corning, and Falcon dishes, set of 6 Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-1425 CC-102 Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
Field Stimulation Chambers
64-0374 RC-47FSLP Quick change chamber for 25 mm coverslips, with field stimulation Login Login
Heater Controllers
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
Valve Control Systems
64-0185 VC-8P Perfusion valve control system, complete, with pinch valves, 8 channel Login Login
64-0135 VC-66CS Perfusion valve control system, complete, with pinch valves, 6 channel Login Login
64-1940 DWV Dedicated Workstation Vacuum Login Login

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