Specialized Tools for Electrophysiology and Cell Biology Research

(DH-40iL) Culture Dish Microincubator

Designed to provide complete environment control for either the Series 40 open bath chambers or 35 mm cell culture dishes.

  • Enables imaging, temperature and atmospheric control within the chamber
  • Sample perfusion supported in both the open and closed configurations
  • Supports Series 40 quick change imaging chambers
  • Supports 35 mm cell culture dishes
  • Compatible with both upright and inverted microscopes
  • Optional heated top coverslip eliminates condensation in the closed configuration

The DH-40iL microincubator is designed for imaging live cells under constant temperature and atmospheric conditions. This compact, versatile incubation system enables high resolution image analysis, fluorescence microscopy, and time-lapse imaging studies while accommodating both upright and inverted microscopes.

The DH-40iL will work, in both its open and closed configurations, with any Warner Series 40 quick change imaging chamber (such as the RC-40LPRC-41LPRC-42LP, and RC-40HP).

In the open configuration, the DH-40iL also works with 35 mm cell culture dishes from Falcon, Corning and Willco, WPI, and MatTek, and can accommodate RC-37 Series dish inserts.

In the closed configuration, the DH-40iL also works with the Corning 35 mm cell culture dish using the new disposable RC-33 Series dish inserts.

Culture Dish Microincubator (DH-40iL)

 

35 MM culture dishes do not all have the same OD and adapter rings are supplied to insure good thermal contact between the dishes and the DH-40iL. Clamps are also provided to insure good contact between the insert and heated surface, and to prevent movement. Both open and closed configurations provide for continuous perfusion of the sample.

The removable top of the incubator is magnetically held in place and has several sealed ports to allow perfusion, aspiration, and gas maintenance. Perfusion and suction tubes can be easily adjusted without disturbing the cell environment. When used in the open configuration, magnetic clamps are supplied to allow entry of perfusion and suction tubes into the unit.

Culture Dish Microincubator (DH-40iL)

 

The base section of the DH-40iL has a 25 mm diameter aperture and the removable top section features a 40 mm glass aperture. An optional heated top coverslip (HSC-1) eliminates condensation when the DH-40iL is used in the closed configuration. Resistive heating of the DH-40iL is provided by means of Warner's TC-344C or TC-324C temperature controllers and a CC-28 cable.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

Culture Dish Microincubator (DH-40iL)

 

The DH-40iL microincubator package includes a set of 4 adapter rings, suction and perfusion tubes, magnetic holders, clamps for Series 40 chambers and 35 mm dishes, PE-160 tubing (10 ft.), 1/16. ID Tygon tubing (25 ft.), and 5 replacement 40 mm coverslips.

The DH-40iL uses Warner's Series 20 stage adapters which are available for all major microscope stages.

 

AR-4 Ring Dimensions

Color Weight ID OD
Gold 1.3 g 38.96 mm 40.62 mm
Green 3.2 g 35.94 mm
Blue 2.7 g 33.81 mm
Red 2.1 g 37.44 mm
Black 3.4 g 35.46 mm
Silver 3.5 g 35.41 mm

Specifications

Weight with Cover 134 g
Weight without Cover 55 g
Order No. Model No. Product Description USA Price Order
64-0107 TA-29 Cable with bead thermistor Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0351 AR-6 Adapter ring kit for DH-35i or QE-1, fits Willco, Corning, and Falcon dishes, set of 6 Login Login
64-1696 CS-40R15 Round cover glass, #1.5 thickness, 40 mm, 50 pack Login Login
64-0102 SH-27B In-line solution heater Login Login
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-1543 HCS-1 Heated top coverslip for DH-35i and DH-40iL chamber Login Login
64-1544 HCS-RPL Replacement ITO coverslip for HCS-1 heated chamber top Login Login
64-1507 RPC-2 Replacement clamps for DH-35i, DH-40iL, QE-1 Login Login
64-2342 GHS-250 Gas humidification system Login Login
64-0103 SF-28 Slow flow in-line solution heater Login Login

References

References

  1. Size-Dependent Cellular Uptake and Expulsion of Single-Walled Carbon Nanotubes: Single Particle Tracking and a Generic Uptake Model for Nanoparticles
    Jin H, Heller DA, Sharma R, Strano MS
    ACS Nano. 2009 Jan 27;3(1):149-58
  2. Multimodal optical sensing and analyte specificity using single-walled carbon nanotubes
    Heller DA, Jin H, Martinez BM, Patel D, Miller BM, Yeung TK, Jena PV, Höbartner C, Ha T, Silverman SK, Strano MS
    Nat Nanotechnol. 2009 Feb;4(2):114-20. Epub 2008 Dec 14
  3. Protein Kinase KIS Localizes to RNA Granules and Enhances Local Translation
    Cambray S, Pedraza N, Rafel M, Garí E, Aldea M, Gallego C
    Mol Cell Biol. 2009 Feb;29(3):726-35. Epub 2008 Nov 17
  4. Cholesterol displacement by ceramide in sphingomyelin-containing liquid-ordered domains, and generation of gel regions in giant lipidic vesicles
    Sot J, Ibarguren M, Busto JV, Montes LR, Goñi FM, Alonso A
    FEBS Lett. 2008 Sep 22;582(21-22):3230-6. Epub 2008 Aug 26
  5. Imaging Clathrin Dynamics in Drosophila melanogaster Hemocytes Reveals a Role for Actin in Vesicle Fission
    Olexiy Kochubey, Amitabha Majumdar and Jurgen Klingauf
    Traffic Volume 7 Issue 12 Page 1614Issue 12 - 1627 - December 2006

Order No. Model No. Product Description USA Price Order
64-0388 DH-40iL Culture dish incubation system Login Login
64-1507 RPC-2 Replacement clamps for DH-35i, DH-40iL, QE-1 Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Accessories
64-2342 GHS-250 Gas humidification system Login Login
Accessories and Replacement Parts
64-1696 CS-40R15 Round cover glass, #1.5 thickness, 40 mm, 50 pack Login Login
64-1543 HCS-1 Heated top coverslip for DH-35i and DH-40iL chamber Login Login
64-1544 HCS-RPL Replacement ITO coverslip for HCS-1 heated chamber top Login Login
64-0351 AR-6 Adapter ring kit for DH-35i or QE-1, fits Willco, Corning, and Falcon dishes, set of 6 Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0102 SH-27B In-line solution heater Login Login
64-0103 SF-28 Slow flow in-line solution heater Login Login
Heater Controllers
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
Valve Control Systems
64-0185 VC-8P Perfusion valve control system, complete, with pinch valves, 8 channel Login Login
64-0135 VC-66CS Perfusion valve control system, complete, with pinch valves, 6 channel Login Login
64-1940 DWV Dedicated Workstation Vacuum Login Login

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