Specialized Tools for Electrophysiology and Cell Biology Research

Culture Dish Incubator (DH-35iL)

Designed to provide complete environmental control over 35 mm culture dishes while maintaining the ability to make both short- and long-term imaging studies.

  • Designed for glass-bottomed 35 mm cell culture dishes
  • Permits imaging, temperature, and gas environment control
  • Compatible with Corning, Falcon, MatTek, Nunc, WillcoWells and WPI Dishes
  • Unique dish clamps permit easy cell access
  • Compatible with Series 40 chambers
  • Compatible with Warner’s RC-37 Cell Culture Dish Perfusion Chamber Inserts

The introduction of glass-bottomed 35 mm culture dishes extended their use into areas such as confocal microscopy, fluorescence microscopy, and high resolution image analysis. The DH-35iL Culture Dish Incubator is a system designed for imaging live cells in an open culture dish (for easy access) or in a closed dish (for gas atmosphere control).

DH-35i Incubators work well with 35 mm culture dishes from all suppliers. Adapter rings are supplied to ensure a close fit for the supported dish. Dishes can be placed in the holder with or without dish covers.

Culture Dish Incubator (DH-35iL)

 

The culture dish holder is machined in three pieces. The midsection and top are easily removed for dish replacement and enables the positioning of electrodes at low angles. Resistive heating of the DH-35iL is provided via the optional Warner TC-344C and TC-324C Temperature Controllers and a CC-28 cable.

Clamps are provided to ensure good contact between the dish and the heated surface and to prevent unwanted movement. The removable midsection is magnetically secured and has several ports to allow multi-channel perfusion, atmospheric maintenance, and aspiration of the dish. The base of the holder has a 25 mm aperture and the removable cover features a 36 mm glass aperture*.

Culture Dish Incubator (DH-35iL)

 

When combined with Warner's RC-37 Cell Culture Perfusion Chamber Insert, a complete micro-environmental system is achieved, and this system is compatible with our SH-27Band SF-28 In-line Solution Heaters. Included with the system are an MP-2 perfusion manifold and a set of 6 culture dish adapter rings. Series 20 Stage adapters are available for all major microscopes. Custom or modified stage adapters are also available; contactour technical support department for details.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

*Distance between bottom and top cover glass is 18.3 mm.

Culture Dish Incubator (DH-35iL)

Specification

AR-4 Ring Dimensions

Color Weight ID OD
Gold 1.3 g 38.96 mm 40.62 mm
Green 3.2 g 35.94 mm
Blue 2.7 g 33.81 mm
Red 2.1 g 37.44 mm
Black 3.4 g 35.46 mm
Silver 3.5 g 35.41 mm
Order No. Model No. Product Description USA Price Order
64-0107 TA-29 Cable with bead thermistor Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0351 AR-6 Adapter ring kit for DH-35i or QE-1, fits Willco, Corning, and Falcon dishes, set of 6 Login Login
64-0348 RC-37F Chamber Insert for Corning, MatTek, Nunc, and Falcon 35 mm cell culture dishes Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-0102 SH-27B In-line solution heater Login Login
64-1696 CS-40R15 Round cover glass, #1.5 thickness, 40 mm, 50 pack Login Login
64-0347 RC-37W Chamber Insert for Willco 35 mm cell culture dishes Login Login
64-1544 HCS-RPL Replacement ITO coverslip for HCS-1 heated chamber top Login Login
64-1507 RPC-2 Replacement clamps for DH-35i, DH-40iL, QE-1 Login Login
64-1455 AR-2 Adapter ring for DH-35i or QE-1, fits Attofluor® cell chamber Login Login
64-2342 GHS-250 Gas humidification system Login Login
64-1543 HCS-1 Heated top coverslip for DH-35i and DH-40iL chamber Login Login
64-0103 SF-28 Slow flow in-line solution heater Login Login

References

References

  1. Extracellular ATP dissociates nonmuscle myosin from P2X7 complex: this dissociation regulates P2X7 pore formation
    Ben J Gu, Catherine Rathsam, Leanne Stokes, Andrew B McGeachie, and James S. Wiley
    Am J Physiol Cell Physiol (June 3, 2009)
  2. Talin phosphorylation by Cdk5 regulates Smurf1-mediated talin head ubiquitylation and cell migration
    Huang C, Rajfur Z, Yousefi N, Chen Z, Jacobson K, Ginsberg MH
    Nat Cell Biol. 2009 May;11(5):624-30. Epub 2009 Apr 12
  3. Single-Cell Transfection by Electroporation Using an Electrolyte/Plasmid-Filled Capillary
    Wang M, Orwar O, Weber SG
    Anal Chem. 2009 May 15;81(10):4060-7
  4. Retinoic Acid-induced Gene-1 (RIG-I) Associates with the Actin Cytoskeleton via Caspase Activation and Recruitment Domain-dependent Interactions
    Mukherjee A, Morosky SA, Shen L, Weber CR, Turner JR, Kim KS, Wang T, Coyne CB
    J Biol Chem. 2009 Mar 6;284(10):6486-94. Epub 2009 Jan 3
  5. Monitoring dynamic protein expression in living E. coli. Bacterial cells by laser tweezers Raman spectroscopy
    Chan JW, Winhold H, Corzett MH, Ulloa JM, Cosman M, Balhorn R, Huser T
    Cytometry A. 2007 Jul;71(7):468-74
  6. Effect of Cell Size and Shape on Single-Cell Electroporation
    Agarwal A, Zudans I, Weber EA, Olofsson J, Orwar O, Weber SG
    Anal Chem. 2007 May 15;79(10):3589-96. Epub 2007 Apr 20
  7. Experimentally Determining the IR Drop in Solution at Carbon Fiber Microelectrodes with Current Interruption and Application to Single-Cell Electroporation
    Lambie BA, Brennan C, Olofsson J, Orwar O, Weber SG
    Anal Chem. 2007 May 15;79(10):3771-8. Epub 2007 Apr 6
  8. Simultaneous maximization of cell permeabilization and viability in single cell electroporation using an electrolyte-filled capillary
    Agarwal A, Zudans I, Orwar O, Weber SG
    Anal Chem. 2007 Jan 1;79(1):161-7

Variations

Order No. Model No. Product Description USA Price Order
64-0347 RC-37W
Chamber Insert for Willco 35 mm cell culture dishes
Login Login
64-0348 RC-37F
Chamber Insert for Corning, MatTek, Nunc, and Falcon 35 mm cell culture dishes
Login Login
64-1455 AR-2
Adapter ring for DH-35i or QE-1, fits Attofluor® cell chamber
Login Login
64-1507 RPC-2
Replacement clamps for DH-35i, DH-40iL, QE-1
Login Login
64-1544 HCS-RPL
Replacement ITO coverslip for HCS-1 heated chamber top
Login Login
64-0351 AR-6
Adapter ring kit for DH-35i or QE-1, fits Willco, Corning, and Falcon dishes, set of 6
Login Login
64-2342 GHS-250
Gas humidification system
Login Login
64-1543 HCS-1
Heated top coverslip for DH-35i and DH-40iL chamber
Login Login
64-1696 CS-40R15
Round cover glass, #1.5 thickness, 40 mm, 50 pack
Login Login
64-1940 DWV
Dedicated Workstation Vacuum
Login Login
64-0185 VC-8P
Perfusion valve control system, complete, with pinch valves, 8 channel
Login Login
64-0135 VC-66CS
Perfusion valve control system, complete, with pinch valves, 6 channel
Login Login
64-0103 SF-28
Slow flow in-line solution heater
Login Login
64-0102 SH-27B
In-line solution heater
Login Login
64-0107 TA-29
Cable with bead thermistor
Login Login
64-0106 CC-28
Cable assembly for heater controllers to platform
Login Login
64-2400 TC-324C
Heater controller, single channel, 110-230 VAC, 50/60Hz
Login Login
64-2401 TC-344C
Heater controller, dual channel, 110-230 VAC, 50/60Hz
Login Login
64-0349 DH-35iL
Culture dish incubation system
Login Login
64-0274 RH-2
Heater elements for Warner chambers and platforms, 2 pack
Login Login

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