Modeled on the RC-21 Series chambers, RC-21BRFS imaging chamber is designed for field stimulation studies.
The RC-21BRFS is a small volume, closed bath imaging chamber designed for field stimulation studies. It uses a pair of 25 mm round coverslips to form the top and bottom of the chamber, and the total bath volume is 263 µL. Two platinum wire electrodes are attached to the parallel sides of the oval-shaped bath. The connecting wires are 15 cm long and are terminated in 1 mm pins.
An additional stainless steel port at the bath input fits a standard Hamilton microliter syringe*. It may be used to inject substances into the flow path or to remove air bubbles from the sealed chamber. At the bath output, a length of tubing is used as a back pressure control to insure smooth perfusate flow.
Field stimulation can be applied via an optional field stimulator.
RC-21BRFS chambers require a platform (PM-2, PH-2 or P-2) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.
Each RC-21BRFS chamber is supplied with a package of #1 thickness glass coverslips (CS-25R, 25 mm diameter, 100/pkg) and 10 ft of PE-160 polyethylene tubing.
* 25 Guage (0.45mm OD) needle
Material | Polycarbonate |
---|---|
Bath dimensions (L x W x H) |
18.0 x 6.3 x 2.3 mm |
Outer dimensions (L x W x H) |
47.5 x 28.5 x 2.5 mm |
Volume | 263 μl |
Electrodes | Platinum wire 15 cm leads with 1 mm pins |
Top coverglass | 25 mm round |
Bottom coverglass | 25 mm round |
Injection port (ID x OD) |
0.91 x 0.56 mm, stainless steel |
Input tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) |
Output tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) |
Order No. | Model No. | Product Description | USA Price | Order |
---|---|---|---|---|
64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
89-0706 | STG4002-1.6mA | Stimulus generator, 2-channel, 4000 Series, ±1.6 mA | Login | Login |
89-0707 | STG4002-160µA | Stimulus generator, 2-channel, 4000 Series, ±160 µA | Login | Login |
89-0708 | STG4002-16mA | Stimulus generator, 2-channel, 4000 Series, ±16 mA | Login | Login |
64-1425 | CC-102 | Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) | Login | Login |
64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
References
Stage Adapters for Series 20 & Series 30 Platforms
PM Series Magnetic Heated Platforms
PH & P Series 20 Platforms
(RC-21B, RC-21BR) Large Closed Bath Imaging Chambers
(STG4000 Series) MCS Stimulus Generators
(DS2A, DS3) Constant Voltage / Constant Current Isolated Stimulators
(DS4) Bipolar Constant Current Isolated Stimulator
D263 Coverslip/Coverglass
Platform Interface Cables, Thermistors, & Replacement Parts
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
Order No. | Model No. | Product Description | USA Price | Order |
---|---|---|---|---|
Chamber | ||||
64-0226 | RC-21BRFS | Imaging chamber with field stimulation | Login | Login |
Platforms | ||||
64-1561 | PM-2 | PM-2 Platform for Series 20 chambers, magnetic clamps, heated | Login | Login |
64-0285 | PH-2 | PH-2 Platform for Series 20 chambers, heater | Login | Login |
64-0278 | P-2 | P-2 Platform for Series 20 chambers, non-heater | Login | Login |
Stimulators - Bipolar | ||||
89-0707 | STG4002-160µA | Stimulus generator, 2-channel, 4000 Series, ±160 µA | Login | Login |
89-0706 | STG4002-1.6mA | Stimulus generator, 2-channel, 4000 Series, ±1.6 mA | Login | Login |
89-0708 | STG4002-16mA | Stimulus generator, 2-channel, 4000 Series, ±16 mA | Login | Login |
64-1425 | CC-102 | Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) | Login | Login |
65-0364 | DS4 | Bipolar stimulator, constant current, isolated | Login | Login |
Stimulators - Monopolar | ||||
89-0707 | STG4002-160µA | Stimulus generator, 2-channel, 4000 Series, ±160 µA | Login | Login |
89-0706 | STG4002-1.6mA | Stimulus generator, 2-channel, 4000 Series, ±1.6 mA | Login | Login |
89-0708 | STG4002-16mA | Stimulus generator, 2-channel, 4000 Series, ±16 mA | Login | Login |
64-1425 | CC-102 | Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) | Login | Login |
65-0550 | DS3 | Monopolar stimulator, constant current, isolated | Login | Login |
65-0329 | DS2A-MKII | Monopolar stimulator, constant voltage, isolated | Login | Login |
Accessories | ||||
64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |
Replacement Consumables | ||||
64-0705 | CS-25R | Round cover glass, #1 thickness, 25 mm, 100 pack | Login | Login |
64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
Temperature Control | ||||
64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
Replacement Parts | ||||
64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |
64-1506 | RPC-1 | Replacement platform clamps and screws for P and PH Series platforms, 2 pack | Login | Login |