Specialized Tools for Electrophysiology and Cell Biology Research

(RC-21BRFS) Slotted Bath with Field Stimulation

Modeled on the RC-21 Series chambers, RC-21BRFS imaging chamber is designed for field stimulation studies.

  • Well optimized for studies with cardiac myocytes
  • Field stimulation chamber
  • Closed bath design
  • Small volume with slotted bath
  • Ideal for fluorescent, calcium and time-lapse imaging studies                               

The RC-21BRFS is a small volume, closed bath imaging chamber designed for field stimulation studies. It uses a pair of 25 mm round coverslips to form the top and bottom of the chamber, and the total bath volume is 263 µL. Two platinum wire electrodes are attached to the parallel sides of the oval-shaped bath. The connecting wires are 15 cm long and are terminated in 1 mm pins.

An additional stainless steel port at the bath input fits a standard Hamilton microliter syringe*. It may be used to inject substances into the flow path or to remove air bubbles from the sealed chamber. At the bath output, a length of tubing is used as a back pressure control to insure smooth perfusate flow.

Field stimulation can be applied via an optional field stimulator.

RC-21BRFS chambers require a platform (PM-2PH-2 or P-2) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.

Slotted Bath with Field Stimulation (RC-21BRFS)
Slotted Bath with Field Stimulation (RC-21BRFS)
64-1561_PM-2
Large Closed Bath Imaging Chambers (RC-21B, RC-21BR)

 

Each RC-21BRFS chamber is supplied with a package of #1 thickness glass coverslips (CS-25R, 25 mm diameter, 100/pkg) and 10 ft of PE-160 polyethylene tubing.

* 25 Guage (0.45mm OD) needle

Material Polycarbonate
Bath dimensions
(L x W x H)
18.0 x 6.3 x 2.3 mm
Outer dimensions
(L x W x H)
47.5 x 28.5 x 2.5 mm
Volume 263 μl
Electrodes Platinum wire
15 cm leads with 1 mm pins
Top coverglass 25 mm round
Bottom coverglass 25 mm round
Injection port
(ID x OD)
0.91 x 0.56 mm, stainless steel
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Output tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)

Order No. Model No. Product Description USA Price Order
64-0705 CS-25R Round cover glass, #1 thickness, 25 mm, 100 pack Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
89-0706 STG4002-1.6mA Stimulus generator, 2-channel, 4000 Series, ±1.6 mA Login Login
89-0707 STG4002-160µA Stimulus generator, 2-channel, 4000 Series, ±160 µA Login Login
89-0708 STG4002-16mA Stimulus generator, 2-channel, 4000 Series, ±16 mA Login Login
64-1424 SIU-102 Bipolar stimulator, high current, non-isolated Login Login
64-1425 CC-102 Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) Login Login
64-0107 TA-29 Cable with bead thermistor Login Login

References

References

  1. The Phospho-Dependent Dynamin–Syndapin Interaction Triggers Activity-Dependent Bulk Endocytosis of Synaptic Vesicles
    Clayton EL, Anggono V, Smillie KJ, Chau N, Robinson PJ, Cousin MA
    J Neurosci. 2009 Jun 17;29(24):7706-17
  2. Superfluous Role of Mammalian Septins 3 and 5 in Neuronal Development and Synaptic Transmission
    Tsang CW, Fedchyshyn M, Harrison J, Xie H, Xue J, Robinson PJ, Wang LY, Trimble WS
    Mol Cell Biol. 2008 Dec;28(23):7012-29. Epub 2008 Sep 22
  3. Two-Photon Fluorescent Probes for Biomembrane Imaging: Effect of Chain Length
    Kim HM, Kim BR, Choo HJ, Ko YG, Jeon SJ, Kim CH, Joo T, Cho BR
    Chembiochem. 2008 Nov 24;9(17):2830-8
  4. Differential labelling of bulk endocytosis in nerve terminals by FM dyes
    Clayton EL, Cousin MA
    Neurochem Int. 2008 Sep;53(3-4):51-5. Epub 2008 Jun 11
  5. Myocardial injection of skeletal myoblasts impairs contractility of host cardiomyocytes
    Giraud MN, Liechti EF, Tchantchaleishvili V, Siepe M, Cook S, Carrel TP, Tevaearai HT
    Int J Cardiol. 2008 Sep 20
  6. Bulk Synaptic Vesicle Endocytosis Is Rapidly Triggered during Strong Stimulation
    Clayton EL, Evans GJ, Cousin MA
    J Neurosci. 2008 Jun 25;28(26):6627-32
  7. Styryl Dye-Based Synaptic Vesicle Recycling Assay in Cultured Cerebellar Granule Neurons
    Anggono V, Cousin MA, Robinson PJ
    Methods Mol Biol. 2008;457:333-45
  8. Physiological Activity Depresses Synaptic Function through an Effect on Vesicle Priming
    Krista L. Moulder, Xiaoping Jiang, Amanda A. Taylor, John W. Olney, and Steven Mennerick
    The Journal of Neuroscience, June 14, 2006, 26(24):6618–6626
  9. Studying vesicle cycling in presynaptic terminals using the genetically encoded probe synaptopHluorin
    Burrone J, Li Z, Murthy VN
    Nat Protoc. 2006;1(6):2970-8
  10. Three modes of synaptic vesicular recycling revealed by single-vesicle imaging
    Gandhi SP, Stevens CF
    Nature. 2003 Jun 5;423(6940):607-13
Order No. Model No. Product Description USA Price Order
64-0226 RC-21BRFS Imaging chamber with field stimulation Login Login
64-1424 SIU-102 Bipolar stimulator, high current, non-isolated Login Login
64-1933 SIU-202 Monopolar stimulator, constant current, isolated Login Login
65-0364 DS4 Bipolar stimulator, constant current, isolated Login Login
65-0329 DS2A-MKII Monopolar stimulator, constant voltage, isolated Login Login
65-0550 DS3 Monopolar stimulator, constant current, isolated Login Login
89-0706 STG4002-1.6mA Stimulus generator, 2-channel, 4000 Series, ±1.6 mA Login Login
89-0707 STG4002-160µA Stimulus generator, 2-channel, 4000 Series, ±160 µA Login Login
89-0708 STG4002-16mA Stimulus generator, 2-channel, 4000 Series, ±16 mA Login Login
64-0705 CS-25R Round cover glass, #1 thickness, 25 mm, 100 pack Login Login
64-1561 PM-2 PM-2 Platform for Series 20 chambers, magnetic clamps, heated Login Login
Accessories and Replacement Parts
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-1425 CC-102 Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
Chambers
64-0226U RC-21BRFS Imaging chamber with field stimulation - polysulfone body Login Login
Heater Controllers
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
Platforms With Heating Elements
64-0285 PH-2 PH-2 Platform for Series 20 chambers, heater Login Login
Platforms Without Heating Elements
64-0278 P-2 P-2 Platform for Series 20 chambers, non-heater Login Login
Valve Control Systems
64-0185 VC-8P Perfusion valve control system, complete, with pinch valves, 8 channel Login Login
64-0135 VC-66CS Perfusion valve control system, complete, with pinch valves, 6 channel Login Login
64-1940 DWV Dedicated Workstation Vacuum Login Login

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