Specialized Tools for Electrophysiology and Cell Biology Research
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(RC-21BRFS) Slotted Bath with Field Stimulation
Modeled on the RC-21 Series chambers, RC-21BRFS imaging chamber is designed for field stimulation studies.
- Well optimized for studies with cardiac myocytes
- Field stimulation chamber
- Closed bath design
- Small volume with slotted bath
- Ideal for fluorescent, calcium and time-lapse imaging studies
The RC-21BRFS is a small volume, closed bath imaging chamber designed for field stimulation studies. It uses a pair of 25 mm round coverslips to form the top and bottom of the chamber, and the total bath volume is 263 µL. Two platinum wire electrodes are attached to the parallel sides of the oval-shaped bath. The connecting wires are 15 cm long and are terminated in 1 mm pins.
An additional stainless steel port at the bath input fits a standard Hamilton microliter syringe*. It may be used to inject substances into the flow path or to remove air bubbles from the sealed chamber. At the bath output, a length of tubing is used as a back pressure control to insure smooth perfusate flow.
Field stimulation can be applied via an optional field stimulator.
RC-21BRFS chambers require a platform (PM-2, PH-2 or P-2) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.
Each RC-21BRFS chamber is supplied with a package of #1 thickness glass coverslips (CS-25R, 25 mm diameter, 100/pkg) and 10 ft of PE-160 polyethylene tubing.
* 25 Guage (0.45mm OD) needle
| Material | Polycarbonate |
|---|---|
| Bath dimensions (L x W x H) |
18.0 x 6.3 x 2.3 mm |
| Outer dimensions (L x W x H) |
47.5 x 28.5 x 2.5 mm |
| Volume | 263 μl |
| Electrodes | Platinum wire 15 cm leads with 1 mm pins |
| Top coverglass | 25 mm round |
| Bottom coverglass | 25 mm round |
| Injection port (ID x OD) |
0.91 x 0.56 mm, stainless steel |
| Input tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) |
| Output tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) |
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| 64-0705 | CS-25R | Round cover glass, #1 thickness, 25 mm, 100 pack | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 89-0706 | STG4002-1.6mA | Stimulus generator, 2-channel, 4000 Series, ±1.6 mA | Login | Login |
| 89-0707 | STG4002-160µA | Stimulus generator, 2-channel, 4000 Series, ±160 µA | Login | Login |
| 89-0708 | STG4002-16mA | Stimulus generator, 2-channel, 4000 Series, ±16 mA | Login | Login |
| 64-1424 | SIU-102 | Bipolar stimulator, high current, non-isolated | Login | Login |
| 64-1425 | CC-102 | Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
References
References
- The Phospho-Dependent Dynamin–Syndapin Interaction Triggers Activity-Dependent Bulk Endocytosis of Synaptic Vesicles
Clayton EL, Anggono V, Smillie KJ, Chau N, Robinson PJ, Cousin MA
J Neurosci. 2009 Jun 17;29(24):7706-17 - Superfluous Role of Mammalian Septins 3 and 5 in Neuronal Development and Synaptic Transmission
Tsang CW, Fedchyshyn M, Harrison J, Xie H, Xue J, Robinson PJ, Wang LY, Trimble WS
Mol Cell Biol. 2008 Dec;28(23):7012-29. Epub 2008 Sep 22 - Two-Photon Fluorescent Probes for Biomembrane Imaging: Effect of Chain Length
Kim HM, Kim BR, Choo HJ, Ko YG, Jeon SJ, Kim CH, Joo T, Cho BR
Chembiochem. 2008 Nov 24;9(17):2830-8 - Differential labelling of bulk endocytosis in nerve terminals by FM dyes
Clayton EL, Cousin MA
Neurochem Int. 2008 Sep;53(3-4):51-5. Epub 2008 Jun 11 - Myocardial injection of skeletal myoblasts impairs contractility of host cardiomyocytes
Giraud MN, Liechti EF, Tchantchaleishvili V, Siepe M, Cook S, Carrel TP, Tevaearai HT
Int J Cardiol. 2008 Sep 20 - Bulk Synaptic Vesicle Endocytosis Is Rapidly Triggered during Strong Stimulation
Clayton EL, Evans GJ, Cousin MA
J Neurosci. 2008 Jun 25;28(26):6627-32 - Styryl Dye-Based Synaptic Vesicle Recycling Assay in Cultured Cerebellar Granule Neurons
Anggono V, Cousin MA, Robinson PJ
Methods Mol Biol. 2008;457:333-45 - Physiological Activity Depresses Synaptic Function through an Effect on Vesicle Priming
Krista L. Moulder, Xiaoping Jiang, Amanda A. Taylor, John W. Olney, and Steven Mennerick
The Journal of Neuroscience, June 14, 2006, 26(24):6618–6626 - Studying vesicle cycling in presynaptic terminals using the genetically encoded probe synaptopHluorin
Burrone J, Li Z, Murthy VN
Nat Protoc. 2006;1(6):2970-8 - Three modes of synaptic vesicular recycling revealed by single-vesicle imaging
Gandhi SP, Stevens CF
Nature. 2003 Jun 5;423(6940):607-13
Related Files
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| 64-0226 | RC-21BRFS | Imaging chamber with field stimulation | Login | Login |
| 64-1424 | SIU-102 | Bipolar stimulator, high current, non-isolated | Login | Login |
| 64-1933 | SIU-202 | Monopolar stimulator, constant current, isolated | Login | Login |
| 65-0364 | DS4 | Bipolar stimulator, constant current, isolated | Login | Login |
| 65-0329 | DS2A-MKII | Monopolar stimulator, constant voltage, isolated | Login | Login |
| 65-0550 | DS3 | Monopolar stimulator, constant current, isolated | Login | Login |
| 89-0706 | STG4002-1.6mA | Stimulus generator, 2-channel, 4000 Series, ±1.6 mA | Login | Login |
| 89-0707 | STG4002-160µA | Stimulus generator, 2-channel, 4000 Series, ±160 µA | Login | Login |
| 89-0708 | STG4002-16mA | Stimulus generator, 2-channel, 4000 Series, ±16 mA | Login | Login |
| 64-0705 | CS-25R | Round cover glass, #1 thickness, 25 mm, 100 pack | Login | Login |
| 64-1561 | PM-2 | PM-2 Platform for Series 20 chambers, magnetic clamps, heated | Login | Login |
| Accessories and Replacement Parts | ||||
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-1425 | CC-102 | Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| 64-1506 | RPC-1 | Replacement platform clamps and screws for P and PH Series platforms, 2 pack | Login | Login |
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |
| Chambers | ||||
| 64-0226U | RC-21BRFS | Imaging chamber with field stimulation - polysulfone body | Login | Login |
| Heater Controllers | ||||
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| Platforms With Heating Elements | ||||
| 64-0285 | PH-2 | PH-2 Platform for Series 20 chambers, heater | Login | Login |
| Platforms Without Heating Elements | ||||
| 64-0278 | P-2 | P-2 Platform for Series 20 chambers, non-heater | Login | Login |
| Valve Control Systems | ||||
| 64-0185 | VC-8P | Perfusion valve control system, complete, with pinch valves, 8 channel | Login | Login |
| 64-0135 | VC-66CS | Perfusion valve control system, complete, with pinch valves, 6 channel | Login | Login |
| 64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |