Specialized Tools for Electrophysiology and Cell Biology Research

(RC-27L) Large Bath Chamber with Slice Supports

Product Summary

A large rectangular bath with a slice support and anchor increases slice viability during long term studies.

  • Designed for tissue slice studies
  • Permits solution flow both above and below a tissue slice
  • Provides good access for immersion optics and electrodes.
  • Can be used with either upright or inverted microscopes
  • Slice anchors available

The RC-27L is designed with tissue slice studies in mind. The tissue slice rests on a slice support and is held in place by a slice anchor (also known as a harp). This design allows the tissue slice to be perfused from both above and below, which can increase the sample.s viability. The slice support rests 0.5 mm above the coverslip floor and are included with the RC-27L.

Slice anchors are sold separately.

The slice support frame is manufactured in polycarbonate and uses fine Lycra threads to suspend the slice. Thread spacing on the slice support is 1 mm.

Slice anchors are available in polycarbonate or stainless steel, and thread spacing on anchors are 1.0, 1.5, and 2.0 mm which allows for maximum access to the preparation. In addition to individual anchors, Warner offers polycarbonate or stainless steel slice anchor kitscontaining anchors in all available thread spacing.

While the weight of a metal harp is sufficient to secure a slice into place, the plastic harp relies on a close apposition between itself and the chamber side walls to maintain its position. This approach can reduce pressure on the slice from the harp.

The RC-27L requires a platform (PM-6DPH-6D or P-6D) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.

 

Each chamber is supplied with an SS-3 slice support, 10 ft of PE-160 polyethylene tubing, and a package of #1 thickness glass coverslips (CS-24/50, 24 x 50 mm, pkg. of 50).

Material Polycarbonate
Bath dimensions
(L x W x H)
38 x 18 x 3 mm
Outer dimensions
(L x W x H)
50 x 25 x 4 - 8 mm
Volume, by depth 560 μl/mm
Bottom coverglass 24 x 50 mm
Slice support 24 x 50 mm, Polycarbonate
Lycra threads at 1 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)


 

Order No. Model No. Product Description USA Price Order
64-0709 CS-24/50 Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0273 SS-3 Standard slice support (1 mm thread spacing) for RC-27L, RC-29 chambers Login Login
64-1533 SS-3G Mesh slice support for RC-27L, RC-29 recording chambers Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. Novel mGluR- and CB1R-Independent Suppression of GABA Release Caused by a Contaminant of the Group I Metabotropic Glutamate Receptor Agonist, DHPG
    Lafourcade CA, Zhang L, Alger BE
    PLoS One. 2009 Jul 1;4(7):e6122
  2. Downregulation of Tonic GABAergic Inhibition in a Mouse Model of Fragile X Syndrome
    Curia G, Papouin T, Séguéla P, Avoli M
    Cereb Cortex. 2009 Jul;19(7):1515-20
  3. Enlarged extracellular space of aquaporin-4–deficient mice does not enhance diffusion of Alexa Fluor 488 or dextran polymers
    Xiao F, Hrabetová S
    Neuroscience. 2009 Jun 16;161(1):39-45
  4. Intracellular Zn2+ increases contribute to the progression of excitotoxic Ca2+ increases in apical dendrites of CA1 pyramidal neurons
    Vander Jagt TA, Connor JA, Weiss JH, Shuttleworth CW
    Neuroscience. 2009 Mar 3;159(1):104-14
  5. Age-Dependent Alterations of Corticostriatal Activity in the YAC128 Mouse Model of Huntington Disease
    Joshi PR, Wu NP, André VM, Cummings DM, Cepeda C, Joyce JA, Carroll JB, Leavitt BR, Hayden MR, Levine MS, Bamford NS
    J Neurosci. 2009 Feb 25;29(8):2414-27
  6. Intracellular Zn2+ Accumulation Contributes to Synaptic Failure, Mitochondrial Depolarization, and Cell Death in an Acute Slice Oxygen–Glucose Deprivation Model of Ischemia
    Medvedeva YV, Lin B, Shuttleworth CW, Weiss JH
    J Neurosci. 2009 Jan 28;29(4):1105-14
  7. Diffusion of flexible random-coil dextran polymers measured in anisotropic brain extracellular space by integrative optical imaging
    Xiao F, Nicholson C, Hrabe J, Hrabetová S
    Biophys J. 2008 Aug;95(3):1382-92
  8. Vasopressin Increases Locomotion through a V1a Receptor in Orexin/Hypocretin Neurons: Implications for Water Homeostasis
    Tsunematsu T, Fu LY, Yamanaka A, Ichiki K, Tanoue A, Sakurai T, van den Pol AN
    J Neurosci. 2008 Jan 2;28(1):228-38
  9. The effects of oleic-acid (OA) on distinct populations of neurons in the hypothalamic arcuate nucleus (ARC) are dependent on extracellular glucose levels
    Wang R, Cruciani-Guglielmacci C, Migrenne S, Magnan C, Cotero VE, Routh VH
    J Neurophysiol. 2006 Mar;95(3):1491-8
  10. Influence of Location of a Fluorescent Zinc Probe in Brain Slices on Its Response to Synaptic Activation
    Alan R. Kay and Katalin Tóth
    J Neurophysiol 95:1949-1956, 2006
  11. Bupivacaine Inhibits Activation of Neuronal Spinal Extracellular Receptor–activated Kinase through Selective Effects on Ionotropic Receptors
    Fumi Yanagidate, M.D., Gary R. Strichartz, Ph.D.
    Anesthesiology 2006; 104:805–14
  12. Cholecystokinin Activates Orexin/Hypocretin Neurons through the Cholecystokinin A Receptor
    Natsuko Tsujino, Akihiro Yamanaka, Kanako Ichiki, Yo Muraki, Thomas S. Kilduff, Ken-ichi Yagami, Satoru Takahashi, Katsutoshi Goto, and Takeshi Sakurai1
    The Journal of Neuroscience, August 10, 2005, 25(32):7459-7469
  13. Reactive oxygen species formation in the transition to hypoxia in skeletal muscle
    Zuo L, Clanton TL
    Am J Physiol Cell Physiol. 2005 Jul;289(1):C207-16
  14. Input of orexin/hypocretin neurons revealed by a genetically encoded tracer in mice
    Sakurai T, Nagata R, Yamanaka A, Kawamura H, Tsujino N, Muraki Y, Kageyama H, Kunita S, Takahashi S, Goto K, Koyama Y, Shioda S, Yanagisawa M
    Neuron. 2005 Apr 21;46(2):297-308
  15. Chronic Cocaine Administration Switches Corticotropin-Releasing Factor2 Receptor-Mediated Depression to Facilitation of Glutamatergic Transmission in the Lateral Septum,
    Jie Liu, Baojian Yu, Luis Orozco-Cabal, Dimitri E. Grigoriadis, Jean Rivier, Wylie W. Vale, Patricia Shinnick-Gallagher, and Joel P. Gallagher,
    The Journal of Neuroscience, January 19, 2005, 25(3):577-583

Order No. Model No. Product Description USA Price Order
Chamber
64-0241 RC-27L Large bath chamber for slice studies, does not include slice hold down Login Login
Platforms
64-1528 PM-6D PM-6D Platform for Series 20 chambers, magnetic clamps, heated Login Login
64-0290 PH-6D PH-6D Platform for Series 20 chambers, heater Login Login
64-0283 P-6D P-6D Platform for Series 20 chambers, non-heater Login Login
Slice Anchors (Harps)
64-0268 SHD-27LKIT Slice anchor kit for RC-27L, RC-27D, RC-29, stainless steel Login Login
64-0256 SHD-27LH/10 Slice anchor for RC-27L, RC-27D, RC-29, 1.0 mm thread spacing, stainless steel Login Login
64-0257 SHD-27LH/15 Slice anchor for RC-27L, RC-27D, RC-29, 1.5 mm thread spacing, stainless steel Login Login
64-0258 SHD-27LH/2 Slice anchor for RC-27L, RC-27D, RC-29, 2.0 mm thread spacing, stainless steel Login Login
Slice Anchors (Harps) - Plastic Frame
64-0190 SHD-27LPKIT Slice anchor kit for RC-27L, RC-27D, RC-29, plastic frame Login Login
64-0259 SHD-27LP/10 Slice anchor for RC-27L, RC-27D, RC-29, 1.0 mm thread spacing, plastic frame Login Login
64-0260 SHD-27LP/15 Slice anchor for RC-27L, RC-27D, RC-29, 1.5 mm thread spacing, plastic frame Login Login
64-0261 SHD-27LP/2 Slice anchor for RC-27L, RC-27D, RC-29, 2.0 mm thread spacing, plastic frame Login Login
Replacement Consumables
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-1533 SS-3G Mesh slice support for RC-27L, RC-29 recording chambers Login Login
64-0273 SS-3 Standard slice support (1 mm thread spacing) for RC-27L, RC-29 chambers Login Login
64-0709 CS-24/50 Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack Login Login
Temperature Control
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Accessories
64-1940 DWV Dedicated Workstation Vacuum Login Login
Replacement Parts
64-1450 CPS-27L Perfusion spreader plate for RC-27L Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
64-3018 PM-plate Pressure plates for PM Series platforms, 2 pack Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login

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