Specialized Tools for Electrophysiology and Cell Biology Research

(RC-26GLP) Low Profile Open Diamond Bath Imaging Chamber

Product Summary

Primarily deigned for studies of cell cultured coverslips.

 

  • Applications include patch clamp, intracellular/extracellular recordings, and imaging
  • Extra-low profile facilitates electrode access
  • Diamond shaped bath provides laminar solution flow
  • Broad optical imaging area
  • Can be used with either upright or inverted microscopes

The RC-26GLP is a low profile version of the RC-26G recording chamber. The sides of this chamber have been milled to a height of only 2 mm and the lower profile provides extra clearance for upright microscopes, water immersion objectives, and permits good electrode access to the chamber bath.

The chamber floor is comprised of a 22 x 40 mm coverslip. The working volume is 180 µl per 1.0 mm of solution height. The chamber is equipped with a stainless steel suction tube together with clamp. The clamp insures the adjustable aspirator tube will not move once the height is set by the user.

The RC-26GLP chamber requires a platform (PM-1PH-1 or P-1) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.

 

Each RC-26GLP chamber is supplied with a package of CS-22/40 glass coverslips (#1 thickness, 22 x 40 mm, 50/pkg) and 10 ft of PE-160 tubing.

 
Material polycarbonate
Bath dimensions
(L x W x H)
24 x 15 x 2 mm
Volume, by depth 180 μl/mm
Bottom coverglass 22 x 40 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)

 

 

 

Order No. Model No. Product Description USA Price Order
64-0707 CS-22/40 Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. Activation of {delta}-Opioid Receptors Reduces Excitatory Input to Putative Gustatory Cells Within the Nucleus of the Solitary Tract
    Zhu M, Cho YK, Li CS
    J Neurophysiol. 2009 Jan;101(1):258-68
  2. Ketamine, But Not Phencyclidine, Selectively Modulates Cerebellar GABAA Receptors Containing {alpha}6 and {delta} Subunits
    Hevers W, Hadley SH, Lüddens H, Amin J
    J Neurosci. 2008 May 14;28(20):5383-93
  3. Preserved BK channel function in vasospastic myocytes from a dog model of subarachnoid hemorrhage
    Jahromi BS, Aihara Y, Ai J, Zhang ZD, Weyer G, Nikitina E, Yassari R, Houamed KM, Macdonald RL
    J Vasc Res. 2008;45(5):402-15. Epub 2008 Apr 10
  4. Synaptogenesis of the Calyx of Held: Rapid Onset of Function and One-to-One Morphological Innervation
    Hoffpauir BK, Grimes JL, Mathers PH, Spirou GA
    J Neurosci. 2006 May 17;26(20):5511-23
  5. Enhancement of long-term memory retention and short-term synaptic plasticity in cbl-b null mice
    Tan DP, Liu QY, Koshiya N, Gu H, Alkon D
    Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5125-30. Epub 2006 Mar 20
  6. Induction of photosensitivity by heterologous expression of melanopsin
    Qiu X, Kumbalasiri T, Carlson SM, Wong KY, Krishna V, Provencio I, Berson DM
    Nature. 2005 Feb 17;433(7027):745-9
  7. In vivo imaging of C. elegans ASH neurons: cellular response and adaptation to chemical repellents
    Hilliard MA, Apicella AJ, Kerr R, Suzuki H, Bazzicalupo P, Schafer WR
    EMBO J. 2005 Jan 12;24(1):63-72
  8. Layer-specific Production of Nitric Oxide during Cortical Circuit Formation in Postnatal Mouse Brain
    Taiko Imura, Shigeaki Kanatani, Satoshi Fukuda, Yusei Miyamoto and Tatsuhiro Hisatsune
    Cerebral Cortex 2005 15(3):332-340
  9. Two Distinct Subpopulations of Nestin-Positive Cells in Adult Mouse Dentate Gyrus
    Fukuda S, Kato F, Tozuka Y, Yamaguchi M, Miyamoto Y, Hisatsune T
    J Neurosci. 2003 Oct 15;23(28):9357-66
  10. Phototransduction by Retinal Ganglion Cells That Set the Circadian Clock
    Berson DM, Dunn FA, Takao M
    Science. 2002 Feb 8;295(5557):1070-3
  11. Melanopsin-Containing Retinal Ganglion Cells: Architecture, Projections, and Intrinsic Photosensitivity
    Hattar S, Liao HW, Takao M, Berson DM, Yau KW
    Science. 2002 Feb 8;295(5557):1065-70

Order No. Model No. Product Description USA Price Order
Chamber
64-0236 RC-26GLP Low profile, large bath recording chamber Login Login
Platforms
64-1526 PM-1 PM-1 Platform for Series 20 chambers, heated, magnetic clamps Login Login
64-0284 PH-1 PH-1 Platform for Series 20 chambers, heater Login Login
64-0277 P-1 P-1 Platform for Series 20 chambers, non-heater Login Login
Slice Anchors (Harps)
64-0266 SHD-26GKIT Slice anchor kit for RC-26G, stainless steel Login Login
64-0253 SHD-26GH/10 Slice anchor for RC-26G, 1.0 mm thread spacing, stainless steel Login Login
64-0254 SHD-26GH/15 Slice anchor for RC-26G, 1.5 mm thread spacing, stainless steel Login Login
64-0255 SHD-26GH/2 Slice anchor for RC-26G, 2.0 mm thread spacing, stainless steel Login Login
Replacement Consumables
64-0707 CS-22/40 Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
Temperature Control
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0102 SH-27B In-line solution heater Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Accessories
64-1940 DWV Dedicated Workstation Vacuum Login Login
Replacement Parts
64-3018 PM-plate Pressure plates for PM Series platforms, 2 pack Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login

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