Specialized Tools for Electrophysiology and Cell Biology Research

RC-26 / RC-26G - Open Diamond Bath Imaging Chambers

Product Summary

Large volume open diamond bath recording chamber that can accommodate large specimens such as slice preparations.

 

  • Applications include patch clamp, intracellular/extracellular recording, and imaging
  • Can be used with tissue slices or cell cultured coverslips
  • Tissue slice anchors available
  • Diamond shaped bath provides laminar solution flow
  • Large viewing area
  • Compatible with upright or inverted microscopy

The RC-26 and RC-26G chambers are designed to be used with cells cultured onto glass coverslips, or with tissue and brain slice samples. The broad imaging area of these chambers (12 x 25 mm and 15 x 24 mm for the RC-26 and RC-26G, respectively) provides excellent access for both electrodes and water-immersion objectives. The diamond shaped geometry facilitates laminar solution flow throughout the bath area.

The large bath of these chambers allows a coverslip to be inserted into the top opening and removed at the end of the experiment. The RC-26 and RC-26G chambers can accept a 12 or 15 mm diameter coverslip, respectively. The working volumes, for 1 mm solution height, of the RC-26 and RC-26G model chambers are 170 and 180 ul, respectively.

 

Large tissue samples can also be placed directly into the chamber. Slice anchor hold downs (harps) are available for tissue slice studies. Anchors are available with thread spacing (Lycra) at 1.0, 1.5, and 2.0 mm.

Both chambers require a platform (PM-1PH-1, or P-1) and Series 20 stage adapter to complete the assembly for mounting on a microscope. The chamber floor consists of a 22 x 40 mm coverslip.

 

 

Each chamber is supplied with a package of #1 thickness glass coverslips (CS-22/40, 22 x 40 mm, 50/pkg) and 10 ft of PE-160 tubing.


An application video is available for this product through the JoVE website!
You can view our selection of videos and access full length videos here.

  RC-26 RC-26G
Material Polycarbonate Polycarbonate
Bath dimensions
(L x W x H)
25 x 12 x 4 mm 24 x 15 x 4 mm
Outer dimensions
(L x W x H)
40 x 23 x 4 mm 40 x 23 x 4 mm
Volume, by depth 150 μl/mm 180 μl/mm
Bottom coverglass 22 x 40 mm 22 x 40 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160) 1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD) 1.67 mm (OD)


Order No. Model No. Product Description USA Price Order
64-0707 CS-22/40 Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. Tumor necrosis factor-[alpha]-induced neutral sphingomyelinase-2 modulates synaptic plasticity by controlling the membrane insertion of NMDA receptors
    Wheeler D, Knapp E, Bandaru VV, Wang Y, Knorr D, Poirier C, Mattson MP, Geiger JD, Haughey NJ
    J Neurochem. 2009 Jun;109(5):1237-49. Epub 2009 Mar 14
  2. Metabolic Acidosis Increases Intracellular Calcium in Bone Cells Through Activation of the Proton Receptor OGR1
    Frick KK, Krieger NS, Nehrke K, Bushinsky DA
    J Bone Miner Res. 2009 Feb;24(2):305-13
  3. Protonophore- and pH-insensitive glucose and sucrose accumulation detected by FRET nanosensors in Arabidopsis root tips
    Chaudhuri B, Hörmann F, Lalonde S, Brady SM, Orlando DA, Benfey P, Frommer WB
    Plant J. 2008 Dec;56(6):948-62. Epub 2008 Sep 18
  4. Deranged Calcium Signaling and Neurodegeneration in Spinocerebellar Ataxia Type 3
    Chen X, Tang TS, Tu H, Nelson O, Pook M, Hammer R, Nukina N, Bezprozvanny I
    J Neurosci. 2008 Nov 26;28(48):12713-24
  5. Evaluation of Dimebon in cellular model of Huntington's disease
    Wu J, Li Q, Bezprozvanny I
    Mol Neurodegener. 2008 Oct 21;3:15
  6. Drosophila Painless Is a Ca2+-Requiring Channel Activated by Noxious Heat
    Sokabe T, Tsujiuchi S, Kadowaki T, Tominaga M
    J Neurosci. 2008 Oct 1;28(40):9929-38
  7. Massive Ca-induced Membrane Fusion and Phospholipid Changes Triggered by Reverse Na/Ca Exchange in BHK Fibroblasts
    Yaradanakul A, Wang TM, Lariccia V, Lin MJ, Shen C, Liu X, Hilgemann DW
    J Gen Physiol. 2008 Jul;132(1):29-50. Epub 2008 Jun 18
  8. Mitochondrial ATP-sensitive K+ channels regulate NMDAR activity in the cortex of the anoxic western painted turtle
    Pamenter ME, Shin DS, Cooray M, Buck LT
    J Physiol. 2008 Feb 15;586(4):1043-58. Epub 2007 Dec 13
  9. Pigment Dispersing Factor-Dependent and -Independent Circadian Locomotor Behavioral Rhythms
    Sheeba V, Sharma VK, Gu H, Chou YT, O'Dowd DK, Holmes TC
    J Neurosci. 2008 Jan 2;28(1):217-27
  10. AMPA receptors undergo channel arrest in the anoxic turtle cortex
    Pamenter ME, Shin DS, Buck LT
    Am J Physiol Regul Integr Comp Physiol. 2008 Feb;294(2):R606-13. Epub 2007 Dec 5
  11. Autocrine activation of EGF receptor promotes oscillation of glutamate-induced calcium increase in astrocytes cultured in rat cerebral cortex,
    Mitsuhiro Morita, Nagisa Kozuka, Rurika Itofusa, Masashi Yukawa, Yosihisa Kudo,
    Journal of Neurochemistry Volume 95 Issue 3 Page 871-879, November 2005
  12. Reelin Modulates NMDA Receptor Activity in Cortical Neurons
    Ying Chen, Uwe Beffert, Mert Ertunc, Tie-Shan Tang, Ege T. Kavalali, Ilya Bezprozvanny, and Joachim Herz1
    The Journal of Neuroscience, September 7, 2005, 25(36):8209-8216
  13. Src regulates distinct pathways for cell volume control through Vav and phospholipase Cgamma,
    Elisabeth T. Barfod, Ann L. Moore, Richard F. Melnick, and Steven D. Lidofsky
    J. Biol. Chem., Vol. 280, Issue 27, 25548-25557, July 8, 2005
  14. Exocytic Trafficking Is Required for Nicotine-induced Up-regulation of 4 2 Nicotinic Acetylcholine Receptors
    Tamara Darsow, T. K. Booker, Juan Carlos PinÜ a-Crespo, and Stephen F. Heinemann
    THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 280, No. 18, Issue of May 6, pp. 18311.18320, 2005
  15. Ileitis modulates potassium and sodium currents in guinea pig dorsal root ganglia sensory neurons
    Stewart T, Beyak MJ, Vanner S
    J Physiol. 2003 Nov 1;552(Pt 3):797-807. Epub 2003 Aug 15

Order No. Model No. Product Description USA Price Order
Chambers
64-0234 RC-26 Large bath recording chamber, 170 µl volume Login Login
64-0235 RC-26G Large bath recording chamber, 234 µl volume Login Login
Platforms
64-1526 PM-1 PM-1 Platform for Series 20 chambers, heated, magnetic clamps Login Login
64-0284 PH-1 PH-1 Platform for Series 20 chambers, heater Login Login
64-0277 P-1 P-1 Platform for Series 20 chambers, non-heater Login Login
Slice Anchors (Harps) - RC-26
64-0265 SHD-26KIT Slice anchor kit for RC-26, stainless steel Login Login
64-0250 SHD-26H/10 Slice anchor for RC-26, 1.0 mm thread spacing, stainless steel Login Login
64-0251 SHD-26H/15 Slice anchor for RC-26, 1.5 mm thread spacing, stainless steel Login Login
64-0252 SHD-26H/2 Slice anchor for RC-26, 2.0 mm thread spacing, stainless steel Login Login
Slice Anchors (Harps) - RC-26G
64-0266 SHD-26GKIT Slice anchor kit for RC-26G, stainless steel Login Login
64-0253 SHD-26GH/10 Slice anchor for RC-26G, 1.0 mm thread spacing, stainless steel Login Login
64-0254 SHD-26GH/15 Slice anchor for RC-26G, 1.5 mm thread spacing, stainless steel Login Login
64-0255 SHD-26GH/2 Slice anchor for RC-26G, 2.0 mm thread spacing, stainless steel Login Login
Replacement Consumables
64-0707 CS-22/40 Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
Temperature Control
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Replacement Parts
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
64-3018 PM-plate Pressure plates for PM Series platforms, 2 pack Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login

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